幸运飞艇官方开户

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文獻解讀

油菜ddRAD遺傳圖定位籽粒芥子油苷含量QTL

FABURIQI:2019-12-24 LIULANCISHU:45

油菜ddRAD遺傳圖定位籽粒芥子油苷含量QTL


摘要

幸运飞艇官方开户 ZHONGZIDIJIEZIYOUGANHANLIANGYIZHISHIYOUCAIGAILIANGDEZHONGYAOYUZHONGMUBIAO。YIYUANSIBEITIYOUCAIDEZHONGZIJIEZIYOUGANHANLIANGBIANHUADEFENZIJIZHIMUQIANSHANGBUQINGCHU。BENWENLIYONGLIANGZHONGXIANGDUIHANLIANGJIAODIDEPINZHONGGOUJIANDHQUNTIJINXINGZHONGZIJIEZIYOUGANHANLIANGQTLDINGWEI,WUZHONGHUANJINGTIAOJIANXIAZAIC02SHANGJIANDINGDAOYIGENENGGOUJIESHI30.88-72.87%BIAOXINGBIANYIDEZHUXIAOQTL,qGSL-C2。LIYONGJINDENGJIYINXI,JIANGqGSL-C2SUODINGDAOWUJINGA02DE49kbGAODUTONGYUANQUDUAN。ZAI5GEHOUXUANJIYINZHONG,BnaC2.MYB28YUNINANJIEMYB28TONGYUAN(BIANMAYIZHONGR2R3-MYBZHUANLUYINZI),JUYOUZHIFANGZUDANANSUANYANSHENGJIEZIYOUGANSHENGWUHECHENGDESHENGWUXUEGONGNENG。XULIEFENXIJIEGUOBIAOMINGJINDENGJIYINXIDEDENGWEIJIYINDEJIYINQUSHANGCUNZAIDUOGEindelHEsnp。JINYIBUFENXIBIAOMINGBnaC2.MYB28DENGWEIJIYINDEBIANYIZAIYOUCAIDEZIRANQUNTIZHONGXIANZHUYUZHONGZIJIEZIYOUGANHANLIANGXIANGGUAN。XITONGFAYUHEXULIEFENXIJIEGUOBIAOMING,LAIZIQINBENG120DEBnaC2.MYB28DENGWEIJIYINYUANYUGANLANBolC2.MYB28,ERLINGYIQINBEN9172GEDENGWEIJIYINKENENGSHITONGGUOTONGYUANJIAOHUANLAIZIWUJINGBraA2.MYB28。

材料方法

YOUCAIZIJIAOXIG120

x 9172,151ZHUDHQUNTI;G120

(GONGTI)x

9172(SHOUTI),BC4F2(6000);454FENYOUCAIZIYUAN(ZIJIAOXI、PINZHONG);37FENWUJINGPINZHONG,18FENGANLANPINZHONG。

幸运飞艇官方开户 CEXUCELVE:DHQUNTI,ddRAD(SacI+MseI)PIANDUANDAXIAO220-500bp,HiSeq

2000 PE90,68,450,311 PE reads。LINGWAIJIASHANG187DUIDUOTAISSRBIAOJI。

幸运飞艇官方开户 TUPUGOUJIAN:JoinMap

4.0,ZUIDAZHONGZUPINLV0.4,ZUIXIAOLOD=3,YICHUANJULI:Kosambi,DUOTAIBIAOJIZAIDHQUNTIZHONGKAFANGJIANYANFUHE1:1FENLIBI。

QTLDINGWEI:WinQTL

幸运飞艇官方开户 cartographer 2.5。

YOUCAIBACWENKU:HBnB(61440GEKELONG,PINGJUN140k,FUGAIJIYINZU9.2x)。

BACXULIEJIYINYUCE:FGENSH

幸运飞艇官方开户 (http://www.softberry.com/)。

JIYINGONGNENGYUCE:JIYINBlast(NCBI,TAIR,BRAD,Genoscope,HDEM)。



研究結果

幸运飞艇官方开户 1、QINBENCAILIAOJIYANSHENGQUNTIDEZHONGZIJIEZIYOUGANHANLIANG

幸运飞艇官方开户 G120(GAO),9172(DI),F1(ZHONGJIANXING),F2FUHE3:1FENLIBI

幸运飞艇官方开户 2、YOUCAIYICHUANTUGOUJIANJIZHONGZIJIEZIYOUHANLIANGQTLDINGWEI

幸运飞艇官方开户 19GELIANSUOQUNGONGBAOHAN910GEsnpHE187GESSRBIAOJI,ZONGTUJU2014.51cM,PINGJUNBIAOJI1.87cM。

SNPZAIJIYINZUSHANGDEFENBU(ZUIWAI:YICHUANTU,DIERQUANWULITU,QICISHI9GEBUTONGHUANJINGqtlDINGWEIJIEGUO,HEISEFANGKUANGBIAOMINGDINGWEIqtlWEIDIAN)C02DINGWEIDAODEQTLZAISUOYOUHUANJINGXIADOUJIANCEDAO,JIESHIBIAOXINGBIANYIWEI30.88-72.87%。JIAXINGXIAOYINGWEI8.33-11.9%

幸运飞艇官方开户 3、ZHONGZIJIEZIYOUHANLIANGQTLJINGXIDINGWEI

LIYONGJINDENGJIYINXIGOUJIANDEBAOHAN6000GEDANZHUDEBC4F2QUNTIDUIDINGWEIQUJIANGONGFENLIBIAOJIJINXINGZHONGZUTISHAIXUAN,ZUIZHONGJIANGHOUXUANJIYINDINGWEIDAOSSA2-146~SSA2-157(49kb)ZHIJIAN,WUJINGJIYINZUDUIYINGQUJIANYOU5GEHOUXUANJIYIN。

幸运飞艇官方开户 QIZHONGBra029311YUNINANJIEMYB28TONGYUAN(BIANMAYIZHONGR2R3-MYBZHUANLUYINZI),JUYOUZHIFANGZUDANANSUANYANSHENGJIEZIYOUGANSHENGWUHECHENGDESHENGWUXUEGONGNENGDANSHIZAIGANLANHEYOUCAIMUQIANCANKAOJIYINZUZHONGWEIZHAODAOGAODUTONGYUANDEXULIE。

幸运飞艇官方开户 LIYONGDINGWEIQUJIANGONGFENLIBIAOJISCAM1HESCAM4DUIYOUCAIDEBACWENKUJINXINGSHAIXUAN,ZUIZHONGHUODE9GEYANGXINGBACDANKELONG。DUIQIZHONGLIANGGEHBnB078K04,HBnB145D12JINXINGCEXU,HUODEMYB28DETONGYUANXULIE。BIJIAOSHUANGQINBnaC2.MYB28XULIEFAXIANZAIJIYINQUHEDIAOKONGQUJUNCUNZAIDUOGEsnpHEindel。

幸运飞艇官方开户 4、BnaC2.MYB28ZIRANBIANYIYUZHONGZIJIEZIYOUGANHANLIANGXIANGGUANXINGFENXI

TIAOXUAN454FENYOUCAIZIYUAN,GENJUHOUXUJIYINFENWEI3ZHONGLEIXING。TypeAHETypeCJIEZIYOUGANHANLIANGXIANZHUGAOYUTypeB。

5、BnaC2.MYB28DENGWEIJIYINKENENGSHIYOUYUTONGYUANZHONGZUERBIAOXIANWEIBUTONGQIYUAN

XITONGFAYUHEJIEGUOBIAOMING,LAIZIQINBENG120DEBnaC2.MYB28DENGWEIJIYINYUANYUGANLANBolC2.MYB28,ERLINGYIQINBEN9172GEDENGWEIJIYINKENENGSHITONGGUOTONGYUANJIAOHUANLAIZIWUJINGBraA2.MYB28。

研究亮點

1、  LIYONGsnpHEssrBIAOJIZAIDUOHUANJINGTIAOJIANXIAJINXINGYOUCAIZHONGZIJIEZIYOUGANHANLIANGQTLJINXINGLEDINGWEI,TONGGUOKUODADEHUIJIAOF2QUNTIJIANGHOUXUANQUJIANSUODINGZAILEWUJINGDUIYINGDE49kTONGYUANQUDUAN。

2、  ZAIWUJINGZHONGZHAODAODEHOUXUANJIYINBINGWEIZAIGANLANHEYOUCAIJIYINZUZHONGZHAODAODUIYINGGAODUTONGYUANDEJIYIN,LIYONGDINGWEIQUJIANJINMILIANSUOBIAOJISHAIXUANYOUCAIBACWENKUHUODEYANGXINGDANKELONG,JINERHUODEYOUCAIZHONGHOUXUANJIYINDEXULIEXINXI。

3、  ZAIZIRANQUNTIZHONGLIYONGHOUXUANJIYINDEDANBEIXINGHEBIAOXINGDEXIANGGUANXING,JINYIBUQUEDINGLEHOUXUANJIYINSHIYUZHONGZIJIEZIYOUGANHANLIANGMIQIEXIANGGUAN。

4、  LIYONGXITONGFAYUSHUFENXITUICECHULIANGQINBENDEMYB28DENGWEIJIYINDEBUTONGLAIYUAN。