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集思慧遠客戶發表《轉錄組學和代謝組學揭示梨果實表皮蠟質調控的分子機制》

FABURIQI:2019-08-29 LIULANCISHU:259

HEZUODANWEI:NANJINGNONGYEDAXUE

幸运飞艇官方开户 YINGXIANGYINZI:IF=3.571


研究背景

       SHUIGUODEBIAOPILACENGYOUFANGZHIJIBING、FANGZHIFEIQIKONGSHUIFENLIUSHIYANCHANGBAOZHIQIDEZHONGYAOZUOYONG。RANER,LI(Pyrus)GUOSHIZHONGBIAOPILASHENGWUHECHENGDEFENZIJICHURENGRANNANYIZHUOMO。

幸运飞艇官方开户        ZHEPIANWENZHANGSHIYONG“YUXIANG”LIGUOSHIDEWUGEFAYUQILAIBIAOZHENGBIAOPILADEBIANHUAJIQIXIANGHUGUANXI,QUANMIANFENXILEJIAOZHILADEXINGTAI、DAIXIEHEZHUANLUTEZHENG,TIGONGLEDIAOKONGJIAOZHILASHENGWUHECHENGDEXIANXINGJIYINDEXINXI。TANTAOLECANYULIGUOSHIJIAOZHILAHECHENG、ZHUANYUNHEDIAOKONGDEGUANJIANJIYINHEDIAOKONGYINZI,WEIJINYIBUDEGONGNENGBIAOZHENGDIANDINGLEJICHU。ZONGHEQILAI,WEIJINHOUDEFENZIYANJIUHEJIYUZHEXIESHUJUJIESHILIGUOSHIJIAOZHILADEYIBANDIAOKONGJIZHITIGONGLEZHONGYAODEJICHU。

實驗設計

       WEILEGENGHAODEYANJIULIGUOSHIZHONGJIAOZHILADESHENGWUHECHENGJIZHI,ZHEPIANWENZHANGCONGXINGTAI、ZHUANLUHEQIXIANGSEPU?ZHIPU(GC-MS)DAIXIEZUXUEJIAODUDUILIGUOSHIFAYUGUOCHENGZHONGDEJIAOZHILASHENGWUHECHENGJINXINGLESHENRUDEYANJIU,CONGERGAISHANLIGUOSHIDEJIAOZHILA。

主要結果

1、GC-MS揭示梨果實發育過程中角質蠟濃度和蠟組分鏈長變化

幸运飞艇官方开户        GC-MSXIANSHIBIAOPILAZAIGUOSHIZAOQI[SHENGHUAHOU20-80TIAN(DAFB)]NONGDUCONG0.64mg/cm2(50 DAFB)ZENGJIADAO1.75mg/cm2,ZAIGUOSHICHENGSHUQI(80-140 DAFB)QIJIAN,LVEWEIJIANGDIZHI1.22mg/cm2。

       LIGUOSHIDEZHUYAOLACHENGFENZAIWUGEFAYUJIEDUANXIANGDANGXIANGSI;ZHEXIECHENGFENZHUYAOBAOKUOWANJING、BOCHUN、ZHIFANGSUAN CID,ZHILEI,QUANLEI,TIELEIHEQITAWEIFENLEIDEHUAHEWU。RANER,BUTONGDEZHUYAOLAZUFENDENONGDUJIQITANLIANCHANGDUQUEYOUHENDADECHAYI。


2、SAOMIAODIANJINGGUANCHALIGUOSHIJIAOZHILADEXINGTAIBIANHUA

       SAOMIAODIANJINGXIANSHI,ZAIGUOSHIFAYUGUOCHENGZHONG,LABANJINGTIZENGDUO,LAJIEGOUFASHENGBIANHUA。


幸运飞艇官方开户 3、BUTONGFAYUJIEDUANYULUXIANGGUOSHIZHONGHOUXUANDEGsDEPCAHEJIANDING

       TONGGUOPCAXUANCHUCHAYIMINGXIANDESANZU(20, 80, and 140 DAFB)JINXINGRNA-SeqFENXI。

       RNA-SeqFENXIDECHUZAI20vs80DAFb、80-140DAFbHE20-140DAFbZHIJIANGONGYOU6977,3302HE6621GEJIYINBEISHANGDIAO,7849,6500HE9736JIYINBEIXIADIAO。ZHONGYAODESHI,ZAIZHEXIEJIYINZHONG,514HE1431GEJIYINZAISUOYOUSANGEFAYUJIEDUANDOUYOUSHANGDIAOHEXIADIAO。


4、DEGsDEGoHEKEGGTONGLUFENXI

       GOZHUSHIJIEGUOBIAOMING,7981GEDEGS(36.20%)BEIBIAOZHUWEI“XIBAOCHENGFEN”;11776GEDEGs(53.42%)BEIBIAOZHUWEI“FENZIGONGNENG”,3194GEDEGS(56.14%)BEIBIAOZHUWEI“SHENGWUGUOCHENG”。TONGGUOGoZHUSHI,SUOYOUDEDEGsBEIHUAFENWEI51GEGONGNENGQU。(TU5A)。CIWAI,GENJUFUJIYINZIHEQZHI,DEGSDEKEGGXIANSHILE6GEGUOCHENGDECANYU:DAIXIE(70.57?,74.49%),YICHUANXINXIGUOCHENG(12.09?18.71%)、HUANJINGXINXIGUOCHENG(4.92?7.07%)、XIBAOGUOCHENG(3.09?3.67%)HESHENGWUXITONG(2.69?3.14%)(ZHICHIXINXIDETUS1)。ZHEXIEJIEGUOBIAOMING,DAIXIESHIZHUYAOTUJING。CIWAI,ZHIFANGDAIXIETUJINGYULASHENGWUHECHENGMIQIEXIANGGUAN。


5、JIYINBIAODAYULACHENGFENDEZONGHEFENXI

幸运飞艇官方开户        ZAIZHUANLUHEGC?MSDAIXIEZUXUESHUJUDEJICHUSHANG,GOUJIANLE8GEZHUYAOLAZUJIANCHAYIJIYINDEGONGBIAODAWANGLUO(WANJING、BOCHUN、ZHIFANGSUAN、TIELEI、ZHILEI、QUAN、TONGHEQITAWEIFENLEIHUAHEWU)。BAOKUO15GEJIEGOUJIYINHE12GEZHUANLUYINZI(TFS)DEJIYINZAINEIDE27GEJIYINZHUYAOYULIGUOSHIBIAOPILAXINGCHENGYOUGUAN(TU6)。ZAIZHE27GEJIYINZHONG,8GEYIQIANWEIBEIBAODAODEJIYINCANYULELAZHISHENGWUHECHENG(Pbr028523,Pbr000672,Pbr000343DENG)JUBAODAO,YOU19BAODAOYUJIAOZHILASHENGWUHECHENGYOUGUAN(PbrKCS 4、PbrLGF 1、PbrW)。


6、YONGqRT-PCRYANZHENGDEGs

幸运飞艇官方开户        ZHUANLUZUJIYINBIAODABIANHUAYUqRT-PCRDEBIANHUAQUSHIGAODUYIZHI。

結論

       JIANLILELIGUOSHIZHONGCHANGLIANZHIFANG(VLCFAs)HEJIAOZHILAHECHENGYUZHUANYUNDEMOXING,WEIYANJIULIGUOSHIZHONGJIAOZHILADESHENGWUHECHENGTIGONGLEYIGEJIZHIKUANGJIA。ZHEXIEJIEGUOWEIYANJIU“YULU”XIANGLIGUOSHIJIAOZHILADEFENZISHIJIANTIGONGLEYIJU,YEYOUZHUYUZHIDAOHOUXUANJIYINDEGONGNENGFENXI。