幸运飞艇官方开户

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集思慧遠客戶發表《在不同的長期耕作和谷物豆類輪作下,根際和根外土壤的細菌和真菌多樣性》

FABURIQI:2019-07-24 LIULANCISHU:482



幸运飞艇官方开户 Bacterial and fungal diversity in rhizosphere and bulk soil under different

long-term tillage and cereal/legume rotation

IF=4.675


ZHAIYAO

幸运飞艇官方开户 TURANGWEISHENGWUDUOYANGXINGDUIBAOCHITURANGZHILIANG、KECHIXUNONGYEHESHENGTAIXITONGGONGNENGJUYOUZHONGYAOYIYI。TURANGGUANLIGONGZUOKEYIYINGXIANGNONGTIANTURANGWEISHENGWUDEDUOYANGXINGHEHUOXING。BENYANJIUDEMUDESHIPINGJIAGENGZUOHELIUCHAGUANLIDUIXIAOMAI-WANDOULUNZUOQIUSHOUQIANGENJIHEGENWAITURANGZHONGXIJUNHEZHENJUNDUOYANGXING、HUAXUEXINGZHIHEZONGTANPAIFANGDEYINGXIANG。CHULIFANGFABAOKUOCHUCHADECHANGGUIGENGZUO(T),CHUCHAMIANGENG(NT),LIUCHADECHANGGUIGENGZUO(TS),LIUCHAMIANGENG(NTS)。ZHENDUIHUNHETUHEGENJITUDEXIJUN16S rRNA(V3V4),ZHENJUNITS(ITS2)JINXINGCEXU。YOUSHIXIJUN(FANGXIANJUN、BIANXINGJUNMEN、LVWANJUNMEN、SUANGANJUNMEN、FUMEIJUNMEN),ZHENJUN(ZINANGJUNMEN、DANZIJUNMEN)ZAIBUTONGCHULIJIANJUNBIAOXIANWEICHAYIBUXIANZHU。GENLIUJUNHEXIYOUDEKESHIDANBAOJIAOMUSHUZAIGENJITUZHONGCUNZAI,GENWAITURANGZHONGBINGWEIJIANCEDAO。CHUCHAMIANGENGHELIUCHAMIANGENGTURANGZHONGXIJUNHEZHENJUNOTUSHULIANGZUIGAO,DANSHIBUTONGCHULIJIANWEISHENGWUQUNLUOQUEMEIYOUCHAYI。GANGSHUIPINGXIJUNHEZHENJUNFENGDUYUTURANGPH,ZONGYOUJITAN,ZONGDAN,ANTAIDANHEXIAOTAIDANHANLIANGDOUSHIXIANGGUANDE。JIEGUOBIAOMING,GENWAITURANGJUYOUJIAOGAODEWEISHENGWUZHONGLEIFENGDU,YOULIYUXIAYIJIWEISHENGWUQUNLUODEJIANLI。

CAILIAOFANGFA

YANGPINCAIJI:SIZHONGCHULIFANGFA(T\NT\TS\NTS),FENBIEQU0-10\10-30cmTUYANG,GENJITUGE3GESHENGWUXUEZHONGFU(HUNYANGPINGJUNFENCHENG3FEN)。

幸运飞艇官方开户 TURANGLIHUAFENXI:pH,ZONGDAN,ZONGYOUJITAN,TURANGWEISHENGWULIANGTAN,DAN,YOUXIAOLIN,XIAOTAIDAN,ANTAIDAN,TURANGHUXI。

測序方案:16S(V3V4),ITS2;Illumina Hiseq 2500 PE250;測序數據量(27,260- 56,436高質量reads);雙端reads拼接:PANDAseq;嵌合體去除:USEARCH v7.1;OTU聚類:UPARSE;

OTU注釋:UCLUST,SORTMERNA,細菌庫(SILVA),真菌庫(UNITE);α多樣性分析:QIIME;PAST version 2:群落相對豐度可視化,NMDS分析;統計學分析:SPSS version 19.0;


YANJIUJIEGUO

1、TURANGXIJUNHEZHENJUNZUFEN

ZAISUOYOUCHULIYANGPINZHONG,YOUSHIMENSHUIPINGXIJUNFENBIEWEIFANGXIANJUNMEN(32.7–41.9%),BIANXINGJUNMEN(13.9–36.9%),LVWANJUNMEN(4.3–12.7%),SUANGANJUNMEN(3.9–10.9%),FUMEIJUNMEN(6.4–11.4%)(SHANGTUa,b);ERYOUSHIMENSHUIPINGZHENJUNFENBIESHIZINANGJUNMEN(42.2–88.9%),WEIZHIZHENJUN(7.9–22.5%),DANZIJUNMEN(1.1–9.0%),BEIBAOMEIMEN(1.2–3.2%)(XIATUa,b)QIEZINANGJUNMENHEDANZIJUNMENZAIGENJITUZHONGFENGDUSHIGAOYUGENWAITURANG。BUTONGCHULIJIANDEXIJUNHEZHENJUNJUNWEIBIAOXIANCHUXIANZHUCHAYI。JIEGUOBIAOMING,WULUNSHIGENGZUOHUANSHIZUOWUCANLIUCHULI,TURANGZHONGPUBIANCUNZAIDEYOUSHIXIJUNMENHEZHENJUNMENDEQUNLUOZUCHENGDOUSHIPUBIANCUNZAIDE。

幸运飞艇官方开户 a/b.GENWAITURANGZHONGFENGDUtop5SHUSHUIPINGXIJUN(LEINUOKASHIJUNSHU、Solirubrobacter、YABAOGANJUNSHU、RUQIUGANJUNSHU、LIANMEIJUNSHU);c/d.GENWAITURANGZHONGFENGDUtop5SHUSHUIPINGZHENJUN(LIANDAOJUNSHU、Paramyrothecium、QIBANJUNSHU、ZHIDINGBAOSHU、BEIBAOMEISHU);

幸运飞艇官方开户 a.GENJITUFENGDUtop5SHUSHUIPINGXIJUN(GENLIUJUNSHU、LIANMEIJUNSHU、LEINUOKASHIJUNSHU、Pseudarthrobacter、RUQIUGANJUNSHU);b. GENJITUFENGDUtop5SHUSHUIPINGZHENJUN(LIANDAOJUNSHU、MAOKEJUNSHU、QINGMEIJUNSHU、QIBANJUNSHU、KESHIDANBAOJIAOMUSHU)。


2.GENGZUOHELIUCHAGUANLIDUITURANGWEISHENGWUQUNLUODEYINGXIANG

0-10cmTURANGZHONGXIJUNOTUSHULIANG:NTS > T > TS > NT,ZHENJUN:NTS = NT > TS > T,10-30cmTURANGNT、TDEXIJUNHEZHENJUNOTUSHULIANGFENBIEZENGJIA(3.4-9%,4.9-25.3%);ERZAIGENJITUZHONGXIJUNOTUSHULIANG:NTS > TS > T > NT,ZHENJUN:NT > TS > NTS > T。

PERMANOVAFENXIJIEGUOBIAOMING,BENYANJIUCAIYONGDECHULIFANGFADUITURANGXIJUNHEZHENJUNQUNLUOJIEGOUYINGXIANGBUXIANZHU。ANOSIMFENXIJIEGUOBIAOMINGGECHULIJIANXIJUNHEZHENJUNQUNLUOJIEGOUSHIGAODUFENLEIDE。


3.GENGZUOHELIUCHAGUANLIDUITURANGHUAXUEXINGZHIHETANPAIFANGYINGXIANG

GENGZUOXIANZHUYINGXIANGTOC(ZONGYOUJITAN),XIAOTAIDAN,TURANGWEISHENGWULIANGTAN、DAN(GENWAI0-10cmTURANG),YINGXIANGGENWAI10-30cmTURANGDEZONGYOUJIDAN,YOUXIAOLIN,XIAOTAIDAN,TURANGWEISHENGWULIANGTAN。LIANGZHONGBUTONGSHENDUTURANGZHONGGENGZUOHECANCHAHUZUOGUANLIZHIYOUDUITURANGWEISHENGWULIANGTANYOUXIANZHUYINGXIANG。

幸运飞艇官方开户 TURANGDEZHIBIAO(pH, TOC, TN, Olsen P, NH4+ -N, NO3ˉ -N, SMBC , SMBN)ZAI0-10cmTURANGDESUOYOUCHULIZHONGBIAOXIANWEIXIANZHUCHAYI。pHHENO3ˉ -NZAINTSZHONGXIANZHUJIANSHAOERTZHONGXIANZHUSHENGGAO,QITAZHIBIAOZAILIANGZHONGCHULIXIAZHENGHAOXIANGFAN。

幸运飞艇官方开户 ZONGTANSHIFANGLIANG:NTS > NT > TS > T,PINGJUNTURANGHUXI:NTS > NT > T > TS;GENGZUOHECANCHADANDUDUILIANGZHONGZHIBIAOYINGXIANGBUXIANZHU,LIANGZHONGCHULIHUZUOTIAOJIANXIADUILIANGGEZHIBIAOYOUXIANZHUYINGXIANG。


幸运飞艇官方开户 4. TURANGXIJUN、ZHENJUNZUCHENGYUHUAXUEXINGZHIDEGUANXI

幸运飞艇官方开户 TURANGLIHUAXINGZHI,ZONGTANSHIFANGLIANGYUBUTONGSHENDUGENWAITUYUXIJUN、ZHENJUNQUNLUOJIEGOUSHIXIANZHUXIANGGUANDE。NENGGOUJIESHI51.2%–59.9%GANGSHUIPINGDETURANGXIJUNBIANYI(a/b),35.4%-39.8.7%DEZHENJUNBIANYI(c/d)。



幸运飞艇官方开户 JIESHIGENJITU75.3%DEXIJUNBIANYIHE62.5%DEZHENJUNBIANYI。