幸运飞艇官方开户

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文獻解讀

集思慧遠客戶發表《楊樹天冬氨酸轉氨酶(AspAT)基因家族鑒定:氮變動時基因表達變化及酶活性變化》

FABURIQI:2019-06-27 LIULANCISHU:354

Genome-Wide Characterization of AspATs in Populus: Gene Expression Variation and Enzyme Activities in Response to Nitrogen Perturbations

Forests  IF=1.956(ERQU)

DANWEI:NANJINGLINYEDAXUE

摘要:

幸运飞艇官方开户 TIANDONGANSUANZHUANANMEI(AspAt)CUIHUAGUANSUANHECAOSUANZHIZHIJIANKENIDEZHUANANFANYING,SHENGCHENGTIANDONGANSUANHE2-YANGWUERSUAN,ZAISUOYOUXIBAOYOUJITIZHONGFAHUIZHUYAOZUOYONGDEANJISUANSHENGWUHECHENGHEDAN(N)DEWENTAIHETANDAIXIE。AspATDESHENGHUABIAOZHENGYIJINGQUDELEJISHINIANDEJINZHAN,ERAspatJIYINJIAZUBUTONGCHENGYUANDEFENZIHESHENGLITEZHENGRENGRANXIANWEIRENZHI,TEBIESHIZAILINMUZHONG。ZAICI,ZAIHEIMIANMU(MAOBAIYANG)ZHONGSHISHILEDUIAspPATBIANMAJIYINDEGUANGFANDEQUANJIYINZUJIANDING(MUBENZHIWUMOSHIZHONG)。DUIZAIYANGSHUJIYINZUZHONGJIANDINGDAODE10GEAspATJIYIN,JINXINGLEXITONGFAYU、JIYINJIEGOU、RANSETIFENBU、SHUNSHIYUANJIAN、BAOSHOUJIXUHEYAXIBAODINGWEIFENXI。RNA-seqYUqRT-PCRYANZHENGBIAOSHI,PtAspATsXIANSHILEBUTONGDEZUZHITEYIXINGBIAODAMOSHI。JINYIBUYANJIULEYANGSHUZAJIAOWUXINGXINanlin895ZHONGTONGYUANAspATsDESHIKONGBIAODA,BIAOMINGJIYINBIAODAYINYUANKUDONGTAIERYI。NJIEHEJIJIEXINGSHUAILAODUIYANGMUAspATZHUANLUBENDUIDEYINGXIANGBIAOMING:WUZHONGPtAspATsZAIXIEPIANZHONGDESHANGSHENG,TONGSHIGENZHONGDELIUZHONGHUOLIUZHONGYISHANGAspATHANLIANGJIJUXIAJIANG。CIWAI,ZAINGUOLIANGDEGENZHONGGUANCHADAOGENGDUODEAspATsZHUANLUBENMINGXIANJIANSHAO。YINCI,YONGNINGJIAOFACEDINGDANJIESHI,AspATDEHUOXINGSHOUDAOMINGXIANDEYIZHI,CUSHIRENWEIMEIHUOXINGSHINXIEPOXIASHENGCHANGXINGTAIDEYIGEGENGZHIJIEDEZHIBIAO。ZONGSHANGSUOSHU,YINGJIXINHAODEBIAODAPUHEMEIHUOXINGDUIYINGJIXINHAODEYINGXIANGJIESHITEDINGJIYINZAIMUBENZHIWUDANSUHUOQUHEZHONGSUDIAOKONGZHONGDESHENGLIYIYI。


結果

幸运飞艇官方开户 1. YANGSHUJIQITAZHIWUWUZHONGDETIANDONGXIANANZHUANANMEIAspATsQUANJIYINZUJIANDING

幸运飞艇官方开户 GENJUSHUIDAONINANJIEDEAspATsJIYINZAIYANGSHUJIYINZUZHONGJINXINGJIANDING,GONGDEDAO10GEAspATsJIADINGJIYIN

2. YANGSHUAspATsYUQITASHUZHONGDEJINHUAGUANXI

WEILESHENRULEJIEXITONGFAYUGUANXI,ZAILINGWAI11ZHONGZHIWUZHONGJINYIBUJIANDINGLETONGYUANAspATs,QIZHONGBAOKUO7GESHUANGZIXIEZHIWU。ZAIGEZHONGZHIWUWUZHONGZHONGJIANDINGDEAsPATTONGYUANXULIEDEZONGSHUCONG5DAO11BIANHUA(BIAO1)。XITONGFAYUFENXIBIAOMING,10GEPtAspatJULEIWEILIANGGEZHUYAOYAJIAZU,JIIαHEIβ(TU1)。

3.RANSETIDINGWEI、JIYINZUJIEGOUHESHUNSHIZUOYONGYUANJIANFENXI

幸运飞艇官方开户 4.  PtAspATDANBAIDEBAOSHOUJIXUHEJIEGOUYU


5 . PtAspATsZAIBUTONGZUZHIZHONGDEBIAODAPUJIDUIDANDEXIANGYING

WEILESHENRULEJIESHIKONGBIAODAMOSHI,LIYONGZHUANLUSHUJUCHUBUFENXILEYANGSHUPtAspATsDEZHUANLUBEN,FENBIEBAOKUO18ZHONGZUZHIHEWUZHONGWEIGUANXIBAOLEIXING(TU5a,b)。WEIYANZHENGPtAspATsDEZUZHITEYIXINGBIAODA,DUI4ZHONGZUZHI(CHENGSHUXIE、YOUXIE、JINGHEGEN)JINXINGLEqRT-PCRJIANCE。YOUYUJIANCEDAODEBIAODAXINHAOJIAODI,TU5cZHONGMEIYOUBIAOSHICHUPtAspAT 8DEBIAODA。


幸运飞艇官方开户 6.YANGSHUWUXINGXINanlin895ZHONGYANGMUAsPATsDEBIAODA

YANGSHU‘Nanlin 895’SHIYIGEZAZHONGWUXINGXI,YUQITADIFANGZHONGZHIDEYANGSHUPINZHONGXIANGBI,JUYOUYOULIANGDESHENGCHANGXINGNENGHEYICHUANZHUANHUADEKECAOZONGXING。AsPATsZUZHITEYIBIAODAZAILIUGEWEIGUANZUZHIZUZHIZHONGSHOUCIDEDAOZHENGSHI(TU6)。


ZAIYANGSHUNanlin895ZHONGJIANDINGDEYANGMUSHIKONGBIAODAMOSHIDEFAXIAN,JIANGnanlin895JINXINGBUYONGDANNONGDUPEIYANG。ZHENGCHANGDANNONGDU(2mM)WEIDUIZHAOYU0mMHE10mMJINXINGDUIBI。BIAOXINGZAI2、3、4ZHOUHOUKESHI。ZHIDEZHUYIDESHI4ZHOUDEDANJIECHULIHUISHIYUANXIEHEGENDEBIAOXINGBIANHUA(TU7a,b)。RANERYIXIEJIYINDUIDANCHULIBIAODAJIANGDICUSHIWOMENJINYIBUTANJIUQIDUIJIJIESHUAIXIEZIDEBIAODA。YOUQUDESHICHULEAspAT4,DABUFENJIYINZAISANGESHUAILAOJIEDUANBIAODACHENGXIANJIANGDI,SHUOMINGSHUAILAOCHUFADEDANQUESHIZHIJIEDAOZHIAspATBIAODAYIZHI(TU7c)。


幸运飞艇官方开户 7.FEIBIANXINGNINGJIAOZHONGDEMEIHUOXING

幸运飞艇官方开户 WEILEZHENGSHISHANGSHUDANYUANSUDEYINGXIANG,LINGWAITIQUDANBAIJINXINGFEIBIANXINGNINGJIAOCESHIMEIHUO。3TIAOZHUYAODETIAODAIZAININGJIAOZHONGKEJIAN,BIAOMING3ZHONGAspATsZUFEN(TU8a)。WEILEFENXININGJIAOMEIHUOXING,YONGImageJLIANGHUAYOUSETIAODAIXINHAO。RUTU8aSUOSHI,DABUFENZHITIAspATs(Cp)HUOXINGZAICHENGSHUHEYOUNENXIEPIANZHONGBEIJIANCE,TONGSHIXIANLITIDEAspATs(Mt)HUOXINGZHUYAOZAIGENZHONG。XIBAOZHIAspATs(Cp)YUZHITIDECHENGXIANXIANGSIDEFENBUQUSHI,DANQIHUOXINGZAISHUPI、XIEBINGQIAO、JINGXIANGBIJIAORUO;BIAOMINGXIBAOZHITONGGONGMEIZHANZUFENJIAOSHAO(TU8b)。

ZONGJIE:

AsPATQUESHISHIYIZHONGZHONGYAODEZHUANANMEIMEI,CHULEJINJINCUIHUAASPDEXINGCHENGYIWAI,HUANFAHUITEDINGDEZUOYONG,TABEIRENWEISHIDANBAIZHIHECHENGDEZHONGYAOZHONGJIANTI。ZAICI,WOMENTONGGUOQUANJIYINZUQUANJIYINZUFENXI,TIGONGLEFENZIHESHENGLIXINXI。ZONGSHANGSUOSHU,YANGSHUWUXINGXINanlin895ZAINXIEPOXIADE(GONG)BIAODAHEMEIPUDEXINFAXIAN,JIESHILEASPATZAIZHIWUSHENGCHANGHEXIEPOFANYINGZHONGDEDAIXIEHESHENGLIXUEYIYI。WEILAIDEGONGZUOJIANGZHUYANYUGENZONGDAIXIEWUSHENGWUHECHENG,BAOKUOAspJIQIXIANGGUANFENZI,YIJIYUZHIWUJISUXIANGHUYINGXIANG,YILEJIEAspATsZAINHEC.DAIXIEDIAOJIEZHONGDEZUOYONG。 YINCI,TONGGUOGUOBIAODADEBIAOXINGFENXIHECrispr/Cas9DEBIAOXINGFENXI,JIANGYOUMINGXIANDEYOUDAO/YIZHIJIYINBEIBAXIANGYUZHIWUSHENGCHANGHEGEZHONGYINGJIYUANDEGONGNENG, QIEYIXIANZAIQIDONGYANJIU。