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集思慧遠客戶發表《33個罌粟不同組織、發育階段和品種的轉錄組分析》

FABURIQI:2019-06-25 LIULANCISHU:366


Scientific data   2019-05-20

IF=5.305

Transcriptomic profles of 33 opium poppy samples in diferent tissues, growth phases, and cultivars

合作單位:中國藥科大學中藥學院



摘要

幸运飞艇官方开户 YINGSUSHISHIJIESHANGZUIZHONGYAODEYAOYONGZHIWUZHIYI,RENGRANSHIMAFEILEIZHITONGYAODEWEIYISHANGYELAIYUAN。RANER,DUIYINGSUZHONGBENYIKUILINSHENGWUJIAN(BIA)SHENGWUHECHENGDEDIAOKONGJIZHIZHIZHISHENSHAO。ZAICI,WOMENSHOUCIGOUJIANLEYINGSUQUANCHANGDUZHUANLUZUSHUJUJI,BINGYU33GEBUTONGZUZHI、SHENGCHANGJIEDUANHEZAIPEIPINZHONGDERNA-seqSHUJUJIEHE。LongreadCEXUCHANSHENGLE902,140TIAOraw data,YOU55,114FENGAOZHILIANGDEZHUANLUBEN,short-readCEXUCHANSHENG1,923,679,864GEcleandata,PINGJUNQ30LVWEI93%。SUIHOUSHIYONGshort-readsDINGLIANGGAOZHILIANGDEZHUANLUBEN,BINGQIEZAIBUTONGDEYANGPINZHONGMEIGEDANJIYINDEBIAODAYONGFPKMHENGLIANG。ZHEXIESHUJUWEIYINGSUZHUANLUFENXITIGONGLEJICHU,ZHEKENENGYOUZHUYUBUZHUOJIANJIEBIANYITIHEYIXIECANYUBLASHENGWUHECHENGDIAOKONGDEFEIBIANMARNA。TAHUANKEYONGYUJIYINZUZUZHUANGHEZHUSHI,ZHEJIANGYOULIYUXINDEZHUANLUBENSHIBIE。



材料與方法:

植物材料:罌粟野生品種(B1)、經定向育種獲得的其他種質資源分別命名為B2、T、G和Z。采集:5個品種灌漿期的葉片;野生品種幼苗、拔節、抽薹、花期葉片及灌漿期根、莖,每個樣品3個重復。

幸运飞艇官方开户 FANGFA:RNA-seqCEXUPINGTAI: Illumina HiSeq X Ten

SANDAIPINGTAI:PacBio;CAILIAOWEIB1DE21GEYANGPINDERNAHUNHECEXU

BIAsHANLIANGCEDING:GUANJIANGQIXIEPIAN,HPLC


文章主要是對三代數據進行組裝校正及組裝、注釋。對二代數據進行了簡單的差異分析。其主要是為以后研究提供了數據基礎。下圖為本研究實驗設計路線: